This study focuses on rather fundamental research concepts and is therefore further removed from a direct societal impact than translational research. However, this study aims to provide insights into how we can analyse metaproteomic samples with more certainty and accuracy. This is vital if we want to gather truthful and relevant insights from metaproteomic research. This metaproteomic research could then have a real impact on society by influencing all its real-world applications. Metaproteomics has a role in the agricultural sector where it can be used to study the relationship between crops, soil, nutrients, and the microorganisms that inhabit this soil. A very hot topic right now is the nitrogen and phosphorus that is, often excessively, used in the agricultural sector and how we can limit this use while still allowing farmers to fertilise their crops to obtain sufficient yields. Here, metaproteomics could potentially come in with biofertilizers and advanced ways of nutrient cycling using microorganisms. In a more medical context, the human gut microbiome has been linked to many different pathologies such as irritable bowel syndromes, cancer, and autism. If we want to unravel the connection between the human gut microbiome and these pathologies, we need accurate and trustworthy results which could lead to therapies and treatments for these diseases. To conclude, since metaproteomics is such a diverse field with many applications, procuring accurate metaproteomics data could help advance many scientific fields.