Introduction - Soft tissue sarcomas (STS) are rare malignant tumors arising from connective tissues. They have more than 70 subtypes and very few of them are therapeutically targeted. Hardly any drug trials for potential drug therapies are succeeding because in vitro models fail to represent their effects in a precisely enough matter. This research aims to improve the collection and processing of STS in order to establish more standardization in cell culturing and, ultimately, more accurate drug testing in vitro. Material & Method - Two models were investigated: 2D patient-derived tissue cell cultures models and the limitedly investigated 3D patient-derived tissue fragment models (PDTF). First, the patient-derived STS was processed in either cell cultures or tissue fragments. In addition, a comparison of culture media will be made in the PDTF and the cell cultures to evaluate which media supports cell growth and viability the most. Results - Ten patient samples were collected and processed into PDTF. Morphological changes only occur in PDTF from viable tumors. Comparable correlations between the circularity or area with viability could not be established over multiple tumor samples. Comparing the different culture media, showed DMEM F12 the worst viability results but no significant differences were observed between DMEM low glucose, DMEM high glucose and HPLM. In addition, two primary cell cultures were developed. Due to slow growing cell cultures and unsuccessful cell cultures no conclusion can be drawn about the culture media. Conclusion - None of the morphological parameters could sufficiently correlate with the viability of the tissue fragments.