Organoids are a type of 3D tissue culture derived from stem cells, recapitulating the architecture and composition of the tissue of origin. The extracellular matrix (ECM) used to embed and support organoid growth in vitro strongly influences their development. To date, the most used matrix is Matrigel. Matrigel is derived form Engelbreth-Holm-Swarm mouse sarcoma. It is not well-defined due to large heterogeneity and batch-to-batch variability. Furthermore, because it is animal-derived it cannot be used for clinical applications. To have a more standardized alternative to animal-derived ECM, synthetic hydrogels are being developed. Recently, poly(2-alkyl-2-oxazoline) (PAOx) hydrogels gained interest due to their versatile chemistry, allowing for customization of polymer and hydrogel properties. Besides (co)polymerization with different oxazoline monomers, PAOx can be functionalized through post-polymer modification reactions, such as with cell-adhesive peptides.