Hormonal anticonception has been used for over 100 years by women for various reasons including to prevent pregnancy, reduce acne and to prevent painful periods. However, the pill might still cause physiological changes in the female body. In this light, we investigated the influence of hormonal anticonception on the brain perfusion in young women. For this research, thirty women were included: fifteen women with a natural menstrual cycle (NC) and fifteen using hormonal anticonception (HA). By using Arterial Spin Labeling (ASL) MRI, perfusion images were obtained and processed with the BASIL toolset. Thereafter, we compared the absolute perfusion values (in ml/100g/min) between the two groups of women. Results suggested there is a significantly higher perfusion in various regions of interest such as the limbic lobe, cerebellum and occipital lobe in women that use hormonal anticonception. These results indicate that hormonal anticonception might have a confounding effect on the brain perfusion. Further research is needed to assess how hormonal anticonception might influence diagnostic imaging of the brain and how to correct for this.