Biomaterials in tissue engineering (TE) are crucial to recreate a tissue in vitro. Up till this day Matrigel is the go-to ’gold standard’ for growing organoids derived from different tissues. However, its xenogenic and tumor related nature (Engelbreth-Holm-Swarm (EHS) sarcoma tumour cells) and high batch-to-batch variability of Matrigel limit its application for TE. Thus, there is a necessity to develop a substitute of Matrigel, which can potentially be used for artificial tissue implantation. Here, we introduce Gelatin methacryloyl (GelMA) as a promising substitute. Differences in molecular weight and degree of functionality in the GelMA products offer tuneable properties and Young’s modulus, which were demonstrated to be important to control intestinal tissue proliferation and differentiation.