Sterilization and disinfection are measures which everybody encounters in a direct or indirect manner. It is essential that this is always done efficiently and correctly. In this study, we investigated the effectiveness of ultraviolet C (UVC) radiation emitted by light emitting diodes (LEDs) for disinfection of medical instruments. Incorrect disinfection can result in higher numbers of healthcare-associated infections which comes with a high societal and economic cost. More knowledge on the use on UVC-LED devices and the implementation in healthcare setting will benefit the public health. We focussed on the application in healthcare setting, however, these results can in addition be translated into other sectors such as the food industry and water treatment applications. Reducing microbiological contamination of water and food is crucial for our safety and health. Moreover, LEDs are a sustainable and environmentally friendly source to generate UVC radiation compared to traditional UVC sources. In low and middle income countries where standard sterilization and disinfection protocols are not always feasible as they come with a high cost for acquiring and maintaining, and the appropriate resources (electricity and water) need to be available for consistent and effective sterilization. UVC-LED devices could provide an added value in those difficult circumstances. To conclude, gaining more knowledge about UVC-LED disinfection can result in a higher quality of life due to possible implementation in various sectors all over the world.